Industry Workshop

TauSTED Xtend – New tools for gentle live imaging at remarkable nanoscale

Organizer:

Leica

IW Speaker:

John R. Pearson

Abstract:

The goal of scientific research is to understand the workings of nature. Given the complex interplay of biomolecules, molecular machines, and higher-order cellular structures, confocal imaging emerged as a fundamental tool owing to the optical sectioning, sensitivity, and the temporal and spatial resolution capabilities.

Imaging intricate cellular structures at nanoscale resolution while characterizing the dynamics of multiple species in the context of live specimens are emerging avenues followed to shed light on biological processes. With the advent of STED (Stimulated Emission Depletion), researchers have realized the visualization of intracellular structures at the nanoscale, unveiling insights into cellular behavior, interactions, and function.

In this workshop, we will demonstrate how our innovative TauSTED Xtend enables gentle imaging of live and fixed samples at the nanoscale. We will show how advances in our TauSTED1 approach to optical nanoscopy deliver cutting-edge resolution and image quality at low light dose, key to accessing fast nanoscale dynamics of cellular processes. We will also show how fluorescence lifetime information can be used for multiplex imaging of different markers, keeping the nanoscopic resolution.

Reference
1. L. A. J. Alvarez, U. Schwarz, L. Friedrich, J. Fölling, F. Hecht, and M. J. Roberti (2020). Pushing STED beyond its limits with TauSTED. Nat Methods. Doi: d42473-021-00241-0

Keywords:

TauSTED Xtend

Industry Workshop

Meet the enhanced DMi8 Inverted Microscope: Intelligent automation, Spinning Disk and THUNDER

Organizer:

Leica

IW Speaker:

Francisco Porto

Abstract:

As our understanding of cell biology deepens, the demands on microscopy to address challenging research questions are increasing. This is evident in the growing complexity of microscopy workflows and the need for quantitative imaging data that is derived from higher quality images.

The need for rigor and reproducibility when acquiring and analyzing microscopy images means that even slight deviations in workflows can introduce significant errors into your data. Constant manual adjustments, such as optimization of correction collars or maintaining immersion with water objectives can be frustrating, often necessitating repeated experiments. Even after the system is set up, the time-consuming task of finding the right region of your sample at the right time, and navigating between different magnifications, remains.

In this talk, we will introduce the new, enhanced DMi8 inverted microscope and its expanded functionality and intelligent automation, which streamlines complex workflows. We will showcase the patented Adaptive Immersion technology that ensures reliable use of optimal water immersion objectives in live cell experiments through an embedded sensor that maintains immersion, without worrying about evaporation. We will demonstrate how combining this with the new Integrated Sample Finder and fully integrated LAS X Navigator experience creates the ultimate synergistic workflow. You’ll see how to identify key regions of interest (ROIs) from low magnification sample overviews and transition to high-resolution imaging with immersion objectives, all while keeping your sample on the stage and providing spatial context to your data throughout your workflow.

We will also discuss the DMi8’s new advanced technology suite including SmartCORR, which automatically optimizes the objective correction collar settings to match your experiment, significantly improving image quality by reducing spherical aberrations. Additionally, high-speed confocal imaging with the new Spinning Disk Confocal Scanner, combined with technologies such as THUNDER and Aivia AI Image Analysis, offers deeper insights from 3D and live samples.

Don’t miss this exciting session to see how these fundamental improvements allow you focus more on producing quality, publishable data and less on managing your microscope.

Keywords:

Thunder, Spinning Disck

Industry Workshop

Microscope Performance Monitor with the new FLUOVIEW™ FV4000

Organizer:

Evident

IW Speakers:

Dr. Bjoern Sieberer

Senior Application Specialist for Confocal Microscopy

Mr. David Peña

Application Specialist for High-End Light Microscopy

Abstract:

Assessing the status of a microscope and controlling its according performance is critical, especially in a multi-user environment like an imaging core facility. Being able to quickly conclude where unexpected results originate from, and which countermeasures need to be taken, will help to maintain operational status and to act to even prevent breakdowns and malfunctions. In this EVIDENT workshop we will introduce the Fluoview Microscope Performance Monitor, a new software embedded performance assessment tool inclusive specific hardware module that will help to evaluate and monitor the microscope performance.

Keywords:

Fluoview

Industry Workshop

Reviewing Tissue Clearing: Hands-on with Clarification and Imaging

Organizer:

Miltenyi Biotec

IW Speakers:

Luis Muñiz

Imaging Application Spcialist Miltenyi Biotec

Edwin Hernandez

CSIC

Abstract:

Due to their inherent complexity and size, biological samples often present challenges for detailed imaging. Light-sheet microscopy has emerged as a crucial tool for capturing these intricate structures. However, to achieve optimal results, whole-sample tissue clearing is essential for rendering specimens transparent.This workshop provides a comprehensive overview of the latest tissue-clearing techniques, both aqueous and solvent-based, used to prepare samples for light-sheet microscopy. Participants will engage in a hands-on clearing session to learn essential tips and tricks to optimize the process for various sample types. Following the clearing demonstration, attendees will experience live imaging of stained samples using the cutting-edge UltraMicroscope Blaze, featuring its innovative lightspeed acquisition mode. This immersive session is designed to deepen understanding and practical skills in tissue clearing and imaging, equipping researchers with the knowledge to apply these techniques to their own complex biological samples.

Keywords:

Light-sheet

Industry Workshop

The Power of Airyscan 2 for Live Dynamics

Organizer:

Zeiss

IW Speaker:

Dr. Verona Villar

Product Application Specialist at Carl Zeiss Iberia

Abstract:

In the field of molecular imaging, researchers have long been challenged by the inability to easily obtain valuable information about the behavior of molecules of interest. Traditional imaging techniques have been limited in their ability to provide insights into molecular concentration, flow dynamics, and directionality. ZEISS has
eveloped two powerful modules for the confocal microscope LSM9 family, in addition to the long-standing FCS module: Dynamics Profiler, and SpectralRICS. These modules offer researchers unique insights into the behavior of their molecules of interest without the need of understanding complex algorithms or having in-depth knowledge of biophysics.During this Industry Workshop (November 20th at 3:30 pm in Room C2), we will show how ZEISS Dynamics Profiler can uncover molecular concentration, asymmetric diffusion, and flow dynamics of fluorescent proteins in your living samples in a single, easy measurement by employing advanced algorithms in combination with the unique Airyscan 2 detector. Develop a more in-depth profile of the molecules in your current experiments, from cell cultures to organoids to whole organisms.

Keywords:

Industry Workshop

Microscopic precision, reproducibility and versatility. Automation as a harmonisation procedure

Organizer:

Eppendorf

IW Speaker:

Dr. Adolfo Molejón

Abstract:

Automation is fundamental in multiple aspects of microscopy. Forgetting the fine control of lenses, aperture and other hardware features, automated XYZ movement of the stage, or High Content Screening analysis is becoming the norm. However, quality of samples is usually an important topic highlighted. How do you prepare your samples? In this workshop, we wish to discuss a setup for the routine procedures you may have and prove in a live software demo where we will use an example, or a real scenario you face daily and how you can load and run an experimental setup with confidence of harmonisation between users and different experiments.

Keywords:

Industry Workshop

TauSTED Xtend – New tools for gentle live imaging at remarkable nanoscale

Organizer:

Leica

IW Speaker:

John R. Pearson

Abstract:

The goal of scientific research is to understand the workings of nature. Given the complex interplay of biomolecules, molecular machines, and higher-order cellular structures, confocal imaging emerged as a fundamental tool owing to the optical sectioning, sensitivity, and the temporal and spatial resolution capabilities.

Imaging intricate cellular structures at nanoscale resolution while characterizing the dynamics of multiple species in the context of live specimens are emerging avenues followed to shed light on biological processes. With the advent of STED (Stimulated Emission Depletion), researchers have realized the visualization of intracellular structures at the nanoscale, unveiling insights into cellular behavior, interactions, and function.

In this workshop, we will demonstrate how our innovative TauSTED Xtend enables gentle imaging of live and fixed samples at the nanoscale. We will show how advances in our TauSTED1 approach to optical nanoscopy deliver cutting-edge resolution and image quality at low light dose, key to accessing fast nanoscale dynamics of cellular processes. We will also show how fluorescence lifetime information can be used for multiplex imaging of different markers, keeping the nanoscopic resolution.

Reference
1. L. A. J. Alvarez, U. Schwarz, L. Friedrich, J. Fölling, F. Hecht, and M. J. Roberti (2020). Pushing STED beyond its limits with TauSTED. Nat Methods. Doi: d42473-021-00241-0

Keywords:

TauSTED Xtend

Industry Workshop

Meet the enhanced DMi8 Inverted Microscope: Intelligent automation, Spinning Disk and THUNDER

Organizer:

Leica

IW Speaker:

Francisco Porto

Abstract:

As our understanding of cell biology deepens, the demands on microscopy to address challenging research questions are increasing. This is evident in the growing complexity of microscopy workflows and the need for quantitative imaging data that is derived from higher quality images.

The need for rigor and reproducibility when acquiring and analyzing microscopy images means that even slight deviations in workflows can introduce significant errors into your data. Constant manual adjustments, such as optimization of correction collars or maintaining immersion with water objectives can be frustrating, often necessitating repeated experiments. Even after the system is set up, the time-consuming task of finding the right region of your sample at the right time, and navigating between different magnifications, remains.

In this talk, we will introduce the new, enhanced DMi8 inverted microscope and its expanded functionality and intelligent automation, which streamlines complex workflows. We will showcase the patented Adaptive Immersion technology that ensures reliable use of optimal water immersion objectives in live cell experiments through an embedded sensor that maintains immersion, without worrying about evaporation. We will demonstrate how combining this with the new Integrated Sample Finder and fully integrated LAS X Navigator experience creates the ultimate synergistic workflow. You’ll see how to identify key regions of interest (ROIs) from low magnification sample overviews and transition to high-resolution imaging with immersion objectives, all while keeping your sample on the stage and providing spatial context to your data throughout your workflow.

We will also discuss the DMi8’s new advanced technology suite including SmartCORR, which automatically optimizes the objective correction collar settings to match your experiment, significantly improving image quality by reducing spherical aberrations. Additionally, high-speed confocal imaging with the new Spinning Disk Confocal Scanner, combined with technologies such as THUNDER and Aivia AI Image Analysis, offers deeper insights from 3D and live samples.

Don’t miss this exciting session to see how these fundamental improvements allow you focus more on producing quality, publishable data and less on managing your microscope.

Keywords:

Thunder, Spinning Disck

Industry Workshop

Microscope Performance Monitor with the new FLUOVIEW™ FV4000

Organizer:

Evident

IW Speakers:

Dr. Bjoern Sieberer

Senior Application Specialist for Confocal Microscopy

Mr. David Peña

Application Specialist for High-End Light Microscopy

Abstract:

Assessing the status of a microscope and controlling its according performance is critical, especially in a multi-user environment like an imaging core facility. Being able to quickly conclude where unexpected results originate from, and which countermeasures need to be taken, will help to maintain operational status and to act to even prevent breakdowns and malfunctions. In this EVIDENT workshop we will introduce the Fluoview Microscope Performance Monitor, a new software embedded performance assessment tool inclusive specific hardware module that will help to evaluate and monitor the microscope performance.

Keywords:

Fluoview

Industry Workshop

Smart Microscopy

Organizer:

Zeiss

IW Speaker:

Soren Prag

Product and Sales Application Specialist, EMEA/LA, ZEISS RMS

Abstract:

Light microscopes provide a research tool that is universally used across samples and scientific questions. In conventional microscopy, finding regions of interest, capturing them, and making changes to acquisition parameters are time-consuming and involve continuous user intervention.

The evolution on microscope automation and image analysis tools has open a new era, the Smart Microscopy, providing new imaging systems that can automate complex workflows. Smart Microscopy combines automated workflows with real-time image analysis, allowing microscopes to monitor samples and adjust their imaging parameters without human intervention, enhancing efficiency and accuracy of data collection.

During this workshop we will perform a live demonstration on Smart Microscopy with ZEISS Celldicoverer 7, our adaptable automation system for advance workflows.

Keywords:

Industry Workshop

To label, or not to label: Label-free three-dimensional imaging and quantitative analysis of live cells using holotomography

Organizer:

Tomocube

IW Speaker:

Bruno Combettes

Tomocube Inc

Abstract:

Holotomography (HT) has emerged as a useful tool for imaging live specimens without additional pre-treatment such as fixation, staining, and fluorescence excitation. HT can achieve long-term three-dimensional (3D) observation of live specimens for weeks without any cellular damage caused by photoactivation. The high resolution under 150 nm lateral achieved through synthetic numerical aperture provides sufficient spatial information to distinguish various subcellular compartments such as nucleus, nucleoli, mitochondria, lipid droplets, etc.

Furthermore, the measured individual cell data can be analyzed to elucidate the temporal 3D volumetric dynamics with the dry mass information. In this workshop, we will present the latest development of a low-coherence holotomography imaging system, HT-X1, and its numerous applications to different types of biological specimens, ranging from unicellular organisms to multicellular specimens. Several case studies will be introduced, such as 3D visualization of subcellular structures in living microorganisms, real-time quantification of cell volume and subcellular lipid contents, deep learning-based cell type classification, etc. We will also discuss how holotomography can be a versatile tool for various research fields combining downstream molecular analysis, such as live cell biology, immunology, microbiology, organoids, stem cells, and in vitro diagnosis.

Keywords:

Industry Workshop

Accelerating Image Analysis with TruAI Deep Learning Technology

Organizer:

Evident

IW Speakers:

Elisa Escolà

Imaging Specialist

Abstract:

Experiments often require data from microscope images. For accurate image analysis, segmentation is used to extract the target area for analysis from the image. A common segmentation method is to apply thresholds to intensity or color values in the image. Although effective, this method can be time-consuming and affect the state of the sample. Next-generation image analysis methods, such as our cellSens imaging software featuring TruAI technology based on deep learning, reduce the risk of sample deterioration while guaranteeing optimum efficiency and accuracy.

Keywords:

Industry Workshop

Scale up your image analysis with AI ZEISS arivis solutions

Organizer:

Zeiss

IW Speakers:

Delisa García

Business Development Software Solutions

Abstract:

As imaging technologies develop, generation of large volumes of complex imaging data is rapidly becoming the norm. However, acquisition of these datasets is only the first step towards gaining the best possible information from them. Once acquired, these datasets need to be visualised processed and analysed to extract quantitative information out of them. How can these images be analysed efficiently? Can AI help? During this workshop you will have the opportunity to learn how cutting-edge AI-driven technologies and solutions can revolutionize your image analysis and accelerate your research.

Keywords:

Industry Workshop

Label free imaging: image without disturbing your samples. Practical session.

Organizer:

Izasa

IW Speakers:

Jordi Recasens

Eclipse Ji

Zuzana Nováková

Q-Phase

Abstract:

The Eclipse Ji is a complete solution to image and analyze microwell plates easily. The Eclipse Ji automates all acquisition settings (including alignment, LED power, exposure time and focus position) to ensure the quality of the data and performs analyses automatically according to the chosen assay. Join this session to discover how to run a label-free cell-counting assay.

Moreover, discover the powerful software in Q-Phase holographic microscope: observe, segment and analyse the data with high accuracy. QPI images are processed and segmented on-the-fly, providing a complex portfolio of tools for data visualization, subpopulation gating, and multi-parameter data mining. It links quantitative data to images and individual cells, which makes optimizing gates and checking outliers extremely easy and efficient.​

Join us in this live-analysis session to unravel the secrets of label-free technology!

Keywords: